The Lady Lions extended their all-time series lead against the Friars to 8-0 behind Gracie Merkle's monstrous night.
Jess Mruzik's 21 kills and outstanding defensive showing elevated the Nittany Lions to the third round.
Penn State suffered through miscues on both sides of the ball to drop its second consecutive game.
The future Nittany Lions running back dominated on both sides of the ball in his junior year.
Barkley amassed 626 rushing yards and six total touchdowns in November while breaking two Eagles franchise records along the way.
Abdul Carter, Kobe King, Jaylen Reed, and AJ Harris all received placements.