The Nittany Lions moved up two spots following consecutive sweeps over Iowa State and LSU.
The 25-16, 25-11, 25-16 win is Penn State's fifth-straight sweep of the season.
Friday's regular season match was played at a pace fitting for a playoff final. A goal from Ally Schlegel and some breathless defending maintained Penn State's four-game unbeaten streak.
Kaitlyn Hord recorded a match-high 12 kills en route to leading the Nittany Lions past the Cyclones 25-22, 25-16, 25-15.
The Lady Lions could potentially be featured in up to five nationally televised games this season.
Up to nine of Penn State's upcoming games will be broadcast nationally on FS1 or ESPN's host of channels.