The Nittany Lions' six-run second inning was overcome by a fired-up River Hawks squad.
Five players changed their numbers, while 15 saw weight changes.
Barkley will reportedly join the Eagles after spending the first six seasons of his career with the New York Giants.
The Golden Gophers' explosive third period handed the Nittany Lions a crucial loss.
The Cavaliers' three-run fourth inning was too much for the Nittany Lions to overcome.
Dane Dowiak's two goals guided the Nittany Lions to win a close game in Columbus.