Lustig replaced Stacy Collins, who departed for Boise State earlier in the week.
The Nittany Lions' season had touchdowns, sacks, and losses to the nation's top programs.
Dupuis spent the last four seasons as the Old Dominion wide receivers coach.
Sean Clifford, Chris Godwin, and Pat Freiermuth all made an impact for their teams in the playoffs.
Lustig brings over 20 years of coaching experience to Penn State.
The hiring comes after the departure of former special teams coordinator Stacy Collins.